Mosquitoes may not be the first thing most people associate with Greenland, but as arctic temperatures rise we may start hearing more about these pesky critters. Dr. Lauren Culler uses these mosquitoes and the snow-melt ponds they breed in as a fascinating study system to investigate changes in mosquito life cycles within a rapidly changing environment
Ph.D. student, Samuel Ramsey of the vanEngelsdorp Lab took 1st place in the 4th annual 3MT competition on April 5, 2017. After much preparation and making it through the first round of competitions the week prior, he scored the top prize!
Sammy will be awarded $500 and will go on to represent UMD in the International competition in October. Here is some info on the background of the competition but without the winners for this year updated on the site.
Please offer him congratulations when you see him. We are very proud!
Honey bees face a myriad of interacting stress factors including pesticide exposure and poor nutrition in intensive agricultural landscapes. Andrew Garavito spent his masters looking at how these factors interact in real-world landscapes to affect honey bee health. Comparing factors such as pollen diet, pesticide contamination of pollen, and drought stressing of pollen, he gained some interesting insights into what bees face on any given day in the field.
A piece of exciting news to welcome you back from spring break!
Natural threats among the flowers lurk. Dr. T'ai Roulston delves into the somewhat macabre world of bumble bee parasitism by conopid or thick-headed flies.
Tackling challenging and complex questions about ecosystems, Dr. Jamin Dreyer explores how we can better understand natural processes to improve human interactions with the environment.
Ants have been farming for millions of years before humans walked the planet. Learn more about the Sericomyrmex ants that Dr. Ješovnik studies.
"Dr. Arnaud Martin details his research adapting the CRISPR-Cas9 gene editing system to crustaceans and butterflies, providing further evidence that supports previous findings surrounding the use of genetic tool kits found in all animals."
Dr. Leslie Pick has a new book out through Academic Press publishers titled Fly Models of Human Diseases. More information on her book can be found here. Check out the flyer below for more details!
We live in a world full of signals. We are constantly bombarded with exciting new combinations of light, sound, taste, smell, and texture. Ultimately our brains interpret these sensations to form what we call reality. However, as it turns out, human beings are only able to perceive a small slice of reality: there are many smells which, for better or worse, we can never smell, and we can only see a tiny slice of the colors light has to offer (we refer to this small portion of wavelengths as the “visible spectrum”).
As it turns out, flies do not base their behavior on the smell of the fruit: they base it on the smell of the yeast growing on the fruit. When yeasts are removed from fruit, the flies do not know where to go. Dr. Syed’s team took a sample of yeasts abundant in fruit and associated SWD flies and found that SWD choose certain yeasts over others, but why?
To answer this question, they sampled the odors that were produced by his yeasts using solid-phase microextraction (SPME) to see if they were different. They were in fact different – qualitatively and quantitatively - with each yeast isolate producing a very complex and wide range of odorant repertoire. Approaching the point of despair at interpreting all of his data, a statistician swooped in to save the day. The statistician was able to distinguish between the yeasts based solely on the odors they produce.
Read more about Dr. Syed’s Work
Scheidler, N. H., Liu, C., Hamby, K. A., Zalom, F. G., & Syed, Z. (2015). Volatile codes: Correlation of olfactory signals and reception in Drosophila-yeast chemical communication. Scientific reports, 5, 14059.
Hickner, P. V., Rivaldi, C. L., Johnson, C. M., Siddappaji, M., Raster, G. J., & Syed, Z. (2016). The making of a pest: Insights from the evolution of chemosensory receptor families in a pestiferous and invasive fly, Drosophila suzukii. BMC genomics, 17(1), 648.
About the author:
Brian Lovett is a PhD student in Dr. Raymond St. Leger’s Lab studying mycology and genetics in agricultural and vector biology systems. He is currently working on projects analyzing mycorrhizal interactions in agricultural systems, the transcriptomics of malaria vector mosquitoes, and the genomes of entomopathogenic fungi.
At the first Entomology colloquium of the Spring 2017 semester, Dr. Zain Sayed described his work on the agricultural pest spotted wing drosophila. His work unravels how female flies use odors from yeasts growing on fruit to find their mates.
Beginning in the Fall of 2016, the entire Department of Entomology pledged to become a green department . Partnering with the UMD Sustainability's Green Office Program we underwent an extensive audit, evaluation, and received a list of sustainable behaviors we are practicing well and where we have room for improvement.
We have 23 goals to meet, 11 of which we have achieved to become Bronze Certified. So, what actions have we taken so far? Check out our list of accomplishments below!
Ear damage? In this case, we are not talking about listening to music too loud or standing too close to the speakers at a rock concert. Instead, Dr. Galen Dively, Professor Emeritus in the Department of Entomology, has unlocked the mystery shrouding the increased dmanage to ears of corn. Read more about Dively's study here.
The Greek philosopher Heraclitus was channeling his inner taxonomist when he declared, “everything is in flux.” Depending on who you ask, taxonomy as a field precariously teeters between being either the foundation of all other biological sciences or the most esoteric debate topic two scientists can choose. Scientists rely on taxonomists to draw the lines between the species that we study so we can all be on the same page while we study them (imagine trying to plan a trip to the zoo without names for the animals). As Dr. Jason Mottern explained during the last Entomology Department colloquium of 2016, these lines are often drawn in pencil.
Congratulations to the winners of the Spring 2017 Ernest N. Cory Undergraduate Scholarship!
Conservation biological control – a fancy term for the support of beneficial insects in a cropping system to enhance natural pest control - has long been of interest in organic agriculture. Lauren Hunt of the Department of Entomology’s Hooks Lab has been investigating the potential for conservation biological control in organic field corn using partridge pea (Chamaecrista fasciculata) to attract and support insects that feed on and parasitize pests. Many predators and parasitoids, collectively called beneficial insects or natural enemies, need nectar resources offered by flowers to successfully develop and reproduce. Partridge pea offers these nectar resources to insects in the form of flowers and via special glands, termed “extrafloral nectaries” located at the base of the petioles. These nectaries are particularly attractive to wasps known to parasitize a variety of insect pests, including stink bugs, corn earworms, and corn borers – common pests of corn and key pests in Lauren’s study.
Post-doc Christopher Taylor (Hamby Lab), graduate student Veronica Johnson (Hooks Lab), and Professor Emeritus Dr. Galen Dively have a new publication titled, "Assessing the use of antimicrobials to sterilize brown marmorated stink bug egg masses and prevent symbiont acquisition" in Journal of Pest Science. You can read the abstract below and find the full feature here. Congratulations on your achievement!
The kudzu bug (Megacopta cribraria) (Family Plataspidae) (Fig. 1) has become a widespread invasive pest in the southern United States since it was first detected in Georgia in 2009. This small, round bug is closely related to stinkbugs (Pentatomidae) and has plagued soybean farmers under non-treatment and high density conditions with yield losses as high as 59%! It feeds on the soybean plants’ leaves and stems with its piercing-sucking mouthparts, which can affect the number of seeds per pod and overall seed weight. The overall damage is astounding, especially when considering all kudzu bugs in North America originated from a single female bug from Japan. Luckily, many studies have been done to track and monitor this pest, such as those done by Jessica Grant.
It can be a lonely world for a bed bug researcher! Dr. Mark Feldlaufer began his presentation by extending an open invitation to visit his bed bug research lab at the United States Department of Agriculture-Agricultural Research Center in Beltsville, Maryland. He assured us that the offer rarely gets takers, and it is really not surprising, all things considered. As parasites that feed on people and cause itchy welts, bed bugs give people the heebie-jeebies. Just a picture or two of the bed bugs (Fig. 1) was enough to have several attendees visibly cringe, as they imagine the marks and persistent itches that usually follow after bed bug bites. However, Dr. Feldlaufer’s fascinating research, which aims to improve bed bug detection and control, may contribute to a future where everyone can rest assured that there truly are no creatures lurking under the bed.
often do not come in contact with them. It is impractical, dangerous, and against labelled usage, to coat every surface and crack of a home or business in insecticide, so bed bug chemical treatments often fail. The silver bullet control method appears to be heat treatments, but, like silver, heat treatments are expensive. Homes are heated to between 120-140⁰F for several hours to kill all bed bug life stages, which tend to cost anywhere between $2,500-$7,000. Though bed bugs themselves do not discriminate against the rich or poor, our methods of remediation tend to do just that. The most effective way to get rid of bed bugs is unaffordable to many.
In the course of his bed bug research, Dr. Feldlaufer has worked on other projects to help to detect and control bed bugs early in their infestations with a number of students, including our very own Dr. Kevin Ulrich. The team found that bed bugs consistently avoided a common chemical insect repellent, DEET, the main ingredient in most mosquito repellents. They did not, however, respond with greater avoidance to higher dosage. Although this may seem like a quick and easy fix, wearing DEET to bed may just cause bed bugs to merely move on to other surfaces (e.g. the couch). On the other hand, they also found that aldehyde compounds produced by bed bugs elicit a strong attractive response to other adult and immature bed bugs. These aldehydes may be one way bed bugs attract each other to form aggregations (Ulrich et al. 2016), suggesting that the aldehydes may be used to develop an inexpensive and more discreet option to lure and trap bed bugs. Dr. Feldlaufer currently aims to develop and test reduced risk pesticides that show promise in killing bed bugs.
Bed bugs can be a terribly menacing presence in anyone’s home, but thankfully Dr. Feldlaufer has dedicated much of his career to keeping us informed about these insects and their crafty activities. By pairing lures with reduced risk pesticides, Dr. Feldlaufer aims to develop options to safely, affordably, and discreetly ensure we can sleep tight assured that the bed bugs will not bite.
Lit L., Schweitzer J.B., and Oberbauer A.M. 2011. Handler beliefs affect scent detection dog outcomes. Animal Cognition 14: 387.
Pfiester M., Koehler P.G., and Pereira R.M. 2008. Ability of bed bug-detecting canines to locate live bed bugs and viable bed bug eggs. Journal of Economic Entomology 101(4):1389-96.
Ulrich, K.R., Kramer, M. and Feldlaufer, M.F. 2016. Ability of bed bug (Hemiptera: Cimicidae) defensive secretions (E)-2-hexenal and (E)-2-octenal to attract adults of the common bed bug Cimex lectularius. Physiological Entomology 41: 103-110.
Hanna Kahl is a master’s student at University of Maryland in Dr. Cerruti Hooks’ lab researching the effects of red clover living mulch on arthropod pests and pollinators.
Samuel Ramsey is a PhD student at University of Maryland in Dr. Dennis vanEngelsdorp’s lab researching Varroa destructor.
Dr. Dennis vanEngelsdorp is one of two college employees named to Clarivate Analytics' 2016 list of Highly Cited Researchers (HCR). HCR is a comprehensive list of influential individuals in various scientific disciplines. More on the announcement can be found on the College of Computer, Mathematical, and Natural Sciences (CMNS) home page.
Skunk, cilantro, burnt rubber—just a few of the many scents you might associate with the pungent odor produced by stink bugs. Like many animals, these bugs produce a variety of chemical odors (called semiochemicals) that modify the behavior of recipient organisms in different ways. That “stink” that these bugs get their name from is just one example. Stink bugs (family Pentatomidae) represent an extremely diverse family of insects that includes both agricultural pests and beneficial predators. Some of the agricultural pest species can cause millions of dollars in damages to crops. At the USDA Agricultural Research Service in Beltsville, Maryland, Dr. Don Weber studies stink bug semiochemicals in hopes of being able to take advantage of their communication to monitor their populations. In particular, Dr. Weber studies pheromones – one kind of semiochemical used for communication within a species. For example, male stink bugs emit pheromones to attract females, or in other cases, both females and males. In other insects, pheromones can also act to signal danger, food resources, or aggregation sites.
Because semiochemical signals often influence insect behavior, synthetically replicating these scents equips growers with a powerful tool to manage agricultural insect pests. This strategy works most effectively if the chemical composition of the natural odor is identified and isolated, and if the behavior it elicits is fully understood. The invasive brown marmorated stink bug (BMSB) (Halyomorpha halys) and the harlequin bug (Murgantia histrionica) (Figure 1) both respond to traps baited with pheromone lures which can be used in order to monitor levels of these pests to determine whether or not to take action against them with insecticides (Figure 2).
With stink bugs, there are some challenges that researchers such as Dr. Weber have faced in using pheromones as lures. Pheromones released by male stink bugs are not exclusively sex pheromones;females, males and nymphs alike are attracted by the male pheromones. This indicates to researchers that those pheromones might have some of the other roles mentioned above, such as signaling aggregation sites.
Yet another piece to this complicated smell puzzle stems from cross-species attraction, meaning that the deployment of a pheromone by one stink bug species can attract another stink bug species. Scientists can monitor BMSB by using MDT, the aggregation pheromone of a completely different stink bug species (the Asian brown-winged green bug, Plautia stali). However, because the pheromone is most attractive to BMSB in the fall, which is after most crop harvests have finished or already starting, so this is not very helpful for most growers1.
To produce better attractants for BMSB, researchers successfully identified the specific aggregation pheromone produced by BMSB adult males. The pheromone (commonly called murgantiol) is comprised of two different isomers, or molecular conformations, with a specific ratio. This ratio is important to know because there can be variation between species, and even between individual bugs, for what ratio is most attractive. Dr. Weber and colleagues determined which isomer ratio of murgantiol was most attractive separately to BMSB, and then combined this pheromone with MDT to see if a blend of the two was attractive to the bugs. The two pheromones together were much more attractive than either alone – with an added advantage of this blend being attractive to the bugs all season long, unlike MDT by itself. These pheromones also do not need to be extremely pure, which is good news for keeping the cost of lure production low.
Harlequin bug males produce murgantiol as well – so the same types of lures can be used to monitor both pest species, though the two species have different ideal isomer ratios5. Harlequin bugs, however, specialize in feeding on cruciferous plants – unlike BMSB, which are unspecific in their preferred hosts (polyphagous). Harlequin bugs were found by Dr. Weber to also be highly attracted to pheromones mixed with mustard oils, which are chemically derived from their host plants’ defensive compounds6,7. Adding these oils to murgantiol therefore could enhance trap performance for harlequin bugs, much like MDT for BMSB.
While Dr. Weber and his colleagues uncovered a lot of information about stink bug pheromones, there is still room to further our understanding. Now that researchers have evidence for which pheromone blends work most effectively for the two species, there is a lot of fine-tuning to be done about how exactly to use them in traps, and the most economical way to produce them. Dr. Weber and his colleagues also have plans in the works to investigate using pheromones for other pest-combating purposes, such as attracting insect predators of pest species like stink bugs, and genetically engineering plants to produce insect pheromones and act as trap crops. Overall pheromones offer an exciting approach to manage not only stink bugs, but many different pest insects, to better protect our agriculture.
To learn more about Dr. Weber’s research or contact him, visit his USDA homepage. For more information on BMSB or Harlequin bugs, you can visit the University of Maryland Extension pages on BMSB and Harlequin bugs.
Authors: Elizabeth Brandt, Aditi Dubey, Morgan Thompson
However, asymptomatic bees are common and can have either low or high virus loads (de Miranda et al. 2012). The story is complicated by the fact that DWV is very closely related to a series of other RNA viruses, such as Varroa destructor virus-1 (VDV-1). And the story gets even more complicated… because of recombination.
Using next generation gene sequencing, Dr. Ryabov (currently a visiting scientist at the United States Department of Agriculture (USDA) Bee Research Lab in Beltsville, MD) and his colleagues at the University of Warwick, UK, decided to characterize the virus diversity in honey bees. They found that the genome of DWV-like viruses could be divided into three functional parts, or “modules”, any of which were sometimes crossed-over between DWV and VDV. They identified three distinct types of genomes: the 100% DWV genome, and two types of recombinants formed by the association of “modules” from DWV and VDV, which they named VDV-1DVD and VDV-1VVD (Moore et al. 2011).
So what was thought to be a single population of viruses is actually a group of variants (VDV-1DVD, VDV-1VVD and DWV). Dr. Ryabov then compared the levels of each variant in honey bee pupae and associated Varroa mites. They found that individual honey bees were usually infected by a mixture of the three variants. Of the three viruses, the recombinant VDV-1DVD’s levels in honey bee pupae was highly associated with its level in associated mites. This suggests this recombinant is more efficiently transmitted between the mite and the honey bee.
When Varroa acts as a vector for the DWV, it increases the levels of viruses in contaminated colonies, causes deformities in the affected workers (Fig. 2), and overall results in increased risk of mortality of the whole colony. But what remains to be determined is whether this is caused by 1) Varroa amplifying and introducing more virulent strains of the virus and/or by 2) Varroa suppressing the honey bee immune response.
To test those two hypotheses, Dr. Ryabov exposed Varroa-naïve honey bees (collected from a Varroa-free region in Scotland) to DWV either orally (in brood food) or through Varroa mite feeding. They monitored the change in DWV diversity and loads within the host as well as changes in honey bee expressed genes to identify potential antivirus immune responses (Ryabov et al. 2014). They detected changes in expression for a number of genes associated with the immune response of honey bees while in presence of the mites. This suggests that the second hypothesis should be further explored.
This study also showed that in Varroa-free colonies (controls), honey bees had highly diverse DWV, though at low levels. When the bees were infected orally, DWV levels remained low, but the composition of the DWV strains changed compared to the controls. When bees were infected through Varroa, two outcomes would happen. Honey bees either showed low levels of diverse DWV strains, or they developed high levels of a single specific variant of DWV or very closely related variants, even though the infecting mite contained a high diversity of strains. By inoculating honey bees through injections (which simulates Varroa feeding), the researchers observed high levels of replication for the recombinant strains containing VDV-1 derived structural gene block. This suggests that these particular strains have an advantage due to the route of transmission. All of this largely supports the first hypothesis that Varroa amplifies more virulent strains of the virus.
In conclusion, this example of the shift in virulence of the DWV – from a benign and asymptomatic virus to a serious disease – illustrates the importance of the process of recombination in the generation of various strains of viruses, and how the addition of a vector, and a new route of transmission, can increase the impact of a virus by altering the relative composition of its strains.
Meghan McConnell is a Master’s student in Dennis vanEngelsdorp’s Lab. She is currently studying honey bees, with a focus on non-chemical control of Varroa mites.
Nathalie Steinhauer is a PhD candidate working in Dennis vanEngelsdorp’s Lab on honey bee health and management practices. Her projects aims to identify and quantify the effects of risk factors associated with increased colony mortality.
Bowen-Walker, P. L., S. J. Martin, & A. Gunn. 1999. The Transmission of Deformed Wing Virus between Honeybees (Apis mellifera L.) by the Ectoparasitic Mite Varroa jacobsoni Oud. Journal of invertebrate pathology 73(1), 101-106.
Highfield, A. C., A., El Nagar, L. C. Mackinde, M. L. N. Laure, M. J. Hall, S. J. Martin, & D. C. Schroeder. 2009. Deformed wing virus implicated in overwintering honeybee colony losses. Applied and environmental microbiology 75(22), 7212-7220.
de Miranda, J. R., L. Gauthier, M. Ribiere, and Y. P. Chen. 2012. Honey bee viruses and their effect on bee and colony health. In D. Sammataro & J. Yoder (Eds.) Honey bee colony health: challenges and sustainable solutions. CRC Press. Boca Raton. 71-102.
Moore, J; Jironkin, A; Chandler, D; Burroughs, N; Evans, DJ; Ryabov, EV (2011) Recombinants between Deformed wing virus and Varroa destructor virus-1 may prevail in Varroa destructor-infested honeybee colonies. Journal of General Virology, 92(1): 156–161. DOI:10.1099/vir.0.025965-0
Ryabov, EV; Wood, GR; Fannon, JM; Moore, JD; Bull, JC; Chandler, D; Mead, A; Burroughs, N; Evans, DJ (2014) A Virulent Strain of Deformed Wing Virus (DWV) of Honeybees (Apis mellifera) Prevails after Varroa destructor-Mediated, or In Vitro, Transmission. PLoS Pathogens, 10(6): 1–21. DOI:10.1371/journal.ppat.1004230