Congratulations to Entomology graduate students Katie Reding and Minh Le, from the Pick lab, for their publication "Genome editing of the vermilion locus generates a visible eye color marker for Oncopeltus fasciatus" in Scientific Reports. The study used CRISPR/Cas9 genome editing to generate an eye color mutant in Oncopeltus (Of). Bugs homozygous for mutations in the Of-vermillion gene are viable, fertile and have bright red rather than black eyes. This is the first visible, genetic marker for this species, paving the way for development of additional genetic tools in this emerging model system for Hemiptera. In addition, these studies mapped Of-vermillion to the X-chromosome, the first gene to be mapped to a chromosome in this species. Finally, experiments using RNAi showed that knocking down the Oncopeltus ortholog of the rosy gene (encoding xanthine dehydrogenase) in Of-vermillion mutants changed the body color from orange-red to yellow, thus identifying a second candidate gene useful as a visible marker.
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